abstract：:Studies were carried out in a packed-bed column fermentor using coffee husk as substrate in order to verify a relationship between caffeine degradation and the respiration of Aspergillus sp. LPBx. Fermentation conditions were optimized by using factorial design experiments. The kinetic study showed that the caffeine d...

journal_title：Applied biochemistry and biotechnology

authors： Brand D,Pandey A,Rodriguez-Leon JA,Roussos S,Brand I,Soccol CR

更新日期：2002-07-01 00:00:00

abstract：:The present study characterized the ability of a bacterial cutinase to improve the wettability of raw cotton fabrics by specific hydrolysis of the cutin structure of the cuticle. The effect of cutinase was studied alone and in coreaction with pectin lyase. The changes in both the fabric and the reaction fluid were mea...

journal_title：Applied biochemistry and biotechnology

authors： Degani O,Gepstein S,Dosoretz CG

更新日期：2002-07-01 00:00:00

abstract：:Glycopeptides produced by Streptomyces species are the drugs used against beta-lactam drug-resistant staphylococcal infections, and vancomycin is important among them. Increased prevalence of resistant strains increased the usage of vancomycin worldwide and also promoted attempts for indigenous production. The optimum...

journal_title：Applied biochemistry and biotechnology

authors： Padma PN,Rao AB,Yadav JS,Reddy G

更新日期：2002-07-01 00:00:00

abstract：:Sphaerotilus natans is a sheathed bacterium existing in the activated sludge of wastewater treatment plants. It is one of the filamentous bacteria causing the bulking and foaming difficulties of activated sludge. Isolating the strain and culturing it in an axenic environment could not only provide the metabolic knowle...

journal_title：Applied biochemistry and biotechnology

authors： Liu K,Chua H,Lo WH,Lawford H,Yu PH

更新日期：2002-04-01 00:00:00

abstract：:In this work, important results from simulations are presented, showing the potentiality of the molecular distillation process for recovering vitamin E from vegetal oils. Two types of molecular distillators are considered: falling film and centrifugal. The results emphasize the degree of recovery and factors that infl...

journal_title：Applied biochemistry and biotechnology

authors： Batistella CB,Moraes EB,Maciel Filho R,Maciel MR

更新日期：2002-04-01 00:00:00

abstract：:The white-rot fungus Trametes multicolor MB 49 has been identified as an excellent producer of the industrially important enzyme laccase. The formation of extracellular laccase could be considerably stimulated by the addition of Cu(II) to a simple, glycerol-based culture medium. In this study, optimal concentrations o...

journal_title：Applied biochemistry and biotechnology

authors： Hess J,Leitner C,Galhaup C,Kulbe KD,Hinterstoisser B,Steinwender M,Haltrich D

更新日期：2002-04-01 00:00:00

abstract：:The ethanologenic bacteria Escherichia coli strains KO11 and LYO1, and Klebsiella oxytoca strain P2, were investigated for their ability to metabolize furfural. Using high performance liquid chromatography and 13C-nuclear magnetic resonance spectroscopy, furfural was found to be completely biotransformed into furfuryl...

journal_title：Applied biochemistry and biotechnology

authors： Gutiérrez T,Buszko ML,Ingram LO,Preston JF

更新日期：2002-04-01 00:00:00

abstract：:IOGEN Corporation of Ottawa, Canada, has recently built a 40t/d biomass-to-ethanol demonstration plant adjacent to its enzyme production facility. It has partnered with the University of Toronto to test the C6/C5 cofermenta-tion performance characteristics of the National Renewable Energy Labora-tory's metabolically e...

journal_title：Applied biochemistry and biotechnology

authors： Lawford HG,Rousseau JD

更新日期：2002-04-01 00:00:00

abstract：:Large-volume parenteral solutions were submitted to heat treatments after being inoculated with Bacillus stearothermophilus ATCC 7953 (Tr = 121 degrees C) and Bacillus subtilis ATCC 9372 (Tr = 104.5 degrees C) spores. The average decimal reduction time for B. stearothermophilus ranged from a D121 degrees C value of 1....

journal_title：Applied biochemistry and biotechnology

authors： Penna TC,Marques M,Machoshvili IA,Ishii M

更新日期：2002-04-01 00:00:00

abstract：:Polyhydroxyalkanoates, biodegradable plastics with the desired physical and chemical properties of conventional synthetic plastics, are extensively investigated. In this study, specific bacterial strains produced specific copolymers from food waste. Copolymers of HB and HV (poly[3-hydroxybutyrate-co-3-hydroxyvalerate]...

journal_title：Applied biochemistry and biotechnology

authors： Wong PA,Chua H,Lo W,Lawford HG,Yu PH

更新日期：2002-04-01 00:00:00

abstract：:Lignocellulosic biomass has great potential as a cheap feedstock in biological processes to produce biofuels or chemicals; however, dilute acid pretreatment at high temperatures produces undesirable compounds. Toxicity tests were done with inhibitors in standard media, to predict the growth-limiting effects on thermop...

journal_title：Applied biochemistry and biotechnology

authors： Thomasser C,Danner H,Neureiter M,Saidi B,Braun R

更新日期：2002-04-01 00:00:00

abstract：:Cofermentation of glucose, xylose, and arabinose is critical for complete bioconversion of lignocellulosic biomass, such as agricultural residues and herbaceous energy crops, to ethanol. We have previously developed a plasmid-bearing strain of Zymomonas mobilis (206C[pZB301]) capable of cofermenting glucose, xylose, a...

journal_title：Applied biochemistry and biotechnology

authors： Mohagheghi A,Evans K,Chou YC,Zhang M

更新日期：2002-04-01 00:00:00

abstract：:The synthesis of tricaprylin, tricaprin, trilaurin, and trimyristin in a solvent-free system was conducted by mixing a commercial immobilized lipase with the organic reagents (glycerol and fatty acid) in a 20-mL batch reactor with constant stirring. The effects of temperature, fatty acid/glycerol molar ratio, and enzy...

journal_title：Applied biochemistry and biotechnology

authors： Langone MA,Sant'Anna GL Jr

更新日期：2002-04-01 00:00:00

abstract：:The capacity for anaerobic decolorization of a sulfonated azo dye, Congo Red, by a strain of a sulfate-reducing bacterium was evaluated. After optimizing the growth rate of the bacteria on a simple carbon source and terminal electron acceptor pair, lactate and sulfate, respectively, the effect of the dye concentration...

journal_title：Applied biochemistry and biotechnology

authors： Diniz PE,Lopes AT,Lino AR,Serralheiro ML

更新日期：2002-03-01 00:00:00

abstract：:The pH dependencies of electrochemomechanical deformation (ECMD) including the cyclic voltammetry and the expansion ratio in conducting polymers, polyaniline (PANI), and poly(o-methoxyaniline) film were studied to elucidate the mechanisms. It was found that the ECMD is governed by the conformational change of polymer ...

journal_title：Applied biochemistry and biotechnology

authors： Kaneto K,Kaneko M

更新日期：2001-10-01 00:00:00

abstract：:The bienzyme electrodes were fabricated by coimmobilization of lactate oxidase (LOD) and lactate dehydrogenase (LDH) onto electrochemically prepared polyaniline (PANI) films. These PANI/LOD/LDH bienzyme electrodes were shown to provide signal amplification by substrate recycling, making it possible to detect L-lactate...

journal_title：Applied biochemistry and biotechnology

authors： Chaubey A,Pande KK,Pandey MK,Singh VS

更新日期：2001-10-01 00:00:00

abstract：:In this article, we describe a solid-state photoelectrochemical cell for light detection. Safranine-T dye mixed with polyvinyl alcohol (PVA) solution was deposited on a conducting and transparent indium-tin-oxide (ITO)-coated glass that was used as one electrode and another ITO-coated glass as the counterelectrode. A ...

journal_title：Applied biochemistry and biotechnology

authors： Dey SK,Manik NB

更新日期：2001-10-01 00:00:00

abstract：:During glycerol fed-batch fermentation, the process could be divided into multiple equal subintervals, and the feed operation was performed in pulse form at the start of each subinterval. Based on the macrokinetic models, the multipulse feed strategy for both glucose and corn steep slurry was determined by a general n...

journal_title：Applied biochemistry and biotechnology

authors： Xie D,Liu D,Zhu H,Liu T

更新日期：2001-08-01 00:00:00

abstract：:Equipment and procedures were developed to study the conversion of lignocellulosic biomass to carboxylic acids using high-solids countercurrent fermentations. Countercurrent fermentations of cattle manure yielded a rapid fermentation (maximum 2.98 g of total acid/[L x d]) with high acid concentrations (maximum of 32.5...

journal_title：Applied biochemistry and biotechnology

authors： Ross MK,Holtzapple MT

更新日期：2001-05-01 00:00:00

abstract：:A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components in egg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contains a reaction station, tip rack, and an eight-tip pipett...

journal_title：Applied biochemistry and biotechnology

authors： Sato R,Takeyama H,Tanaka T,Matsunaga T

更新日期：2001-04-01 00:00:00

abstract：:A formaldehyde-tolerant bacterium designated as a DM-2 strain was used to biodegrade formaldehyde. The cells, precultivated in the presence of 400 ppm of formaldehyde, were able to degrade formaldehyde in a minimal medium supplemented with up to 400 ppm of formaldehyde in the presence of 3% NaCl. The rate of formaldeh...

journal_title：Applied biochemistry and biotechnology

authors： Yamazaki T,Tsugawa W,Sode K

更新日期：2001-04-01 00:00:00

abstract：:Growth kinetics and red pigment production of Monascus purpureus CCT 3802 was studied. A reproducible inoculum with extremely dispersed hyphae for bioreactor runs was obtained through a two-step cultivation in a shaker. First, the spores were cultivated in a complex medium rendering a suspension of vegetative cells. I...

journal_title：Applied biochemistry and biotechnology

authors： Pereira DG,Kilikian BV

更新日期：2001-04-01 00:00:00

abstract：:A full factorial design leading to 20 sets of sorption runs was conducted to study the influence of four variables (bleaching earth and biomass concentrations, pH, and sorption time) on the iron, nickel, and chromium removal from stainless steel effluent using waste biomass from a beverage industry. Similar factor eff...

journal_title：Applied biochemistry and biotechnology

authors： Dias MA,Rosa CA,Linardi VR,Conte RA,De Castro HF

更新日期：2001-04-01 00:00:00

abstract：:Hydrolysates were obtained from dilute sulfuric acid pretreatment of whole-tree softwood forest thinnings and softwood sawdust. Mid-infrared (IR) spectra were obtained on sample sets of wet washed hydrolysates, and 45 degrees C vacuum-dried washed hydrolysates, using a Fourier transform infrared (FTIR) spectrophotomet...

journal_title：Applied biochemistry and biotechnology

authors： Tucker MP,Nguyen QA,Eddy FP,Kadam KL,Gedvilas LM,Webb JD

更新日期：2001-04-01 00:00:00

abstract：:The enzyme cellobiase from Novo was immobilized in controlled pore silica particles by covalent binding with the silane-glutaraldehyde method with protein and activity yields of 67 and 13.7%, respectively. The activity of the free enzyme (FE) and immobilized enzyme (IE) was determined with 2 g/L of cellobiose, from 40...

journal_title：Applied biochemistry and biotechnology

authors： Calsavara LP,De Moraes FF,Zanin GM

更新日期：2001-04-01 00:00:00

abstract：:Beta-xylosidase recovery by micelles using cetyl trimethyl ammonium bromide (CTAB) cationic surfactant was verified under different experimental conditions. A 2(5-1) fractional factorial design with center points was employed to verify the influence of the following factors on enzyme extraction: pH (x1), CTAB concentr...

journal_title：Applied biochemistry and biotechnology

authors： Hasmann FA,Pessoa A Jr,Roberto IC

更新日期：2001-04-01 00:00:00

abstract：:Enzymes from thermophilic microorganisms, thermozymes, have unique characteristics such as temperature, chemical, and pH stability. They can be used in several industrial processes, in which they replace mesophilic enzymes or chemicals. Thermozymes are often used when the enzymatic process is compatible with existing ...

journal_title：Applied biochemistry and biotechnology

authors： Bruins ME,Janssen AE,Boom RM

更新日期：2001-02-01 00:00:00

abstract：:This article deals with a direct electrochemical method of detecting antigens using new methods of functionalization of gold electrodes. Based on the reacting ability of gold with sulfhydryl groups, three protocols for the fixation of antibodies have been explored. They are based on either the self-assembling properti...

journal_title：Applied biochemistry and biotechnology

authors： Ameur S,Martelet C,Jaffrezic-Renault N,Chovelon JM

更新日期：2000-11-01 00:00:00

abstract：:Two protocols for functionalization of glass supports with hexaethylene glycol (HEG)-linked oligonucleotides were developed. The first method (standard amidite protocol) made use of the 2-cyanoethyl-phosphoramidite derivative of 4,4'-dimethoxytrityl-protected HEG. This was first coupled to the support by standard soli...

journal_title：Applied biochemistry and biotechnology

authors： Sojka B,Piunno PAE,Wust CC,Krull UJ

更新日期：2000-10-01 00:00:00

abstract：:The batch culture of a newly isolated strain of a green microalga, Chlorella sorokiniana, was carried out using a conical helical tubular photobioreactor. The isolate was capable of good growth at 40 degrees C under an airstream enriched with 10% CO2. The maximum photosynthetic productivity was 34.4 g of dry biomass/(...

journal_title：Applied biochemistry and biotechnology

authors： Morita M,Watanabe Y,Saiki H

更新日期：2000-06-01 00:00:00

abstract：:Agar-immobilized alkaliphilic Bacillus sp. AR-009 cells were used for xylanase production using batch and continuous culture. In a batch culture, maximum enzyme production was observed after 48 h and remained high up to 72 h. In repeated batch cultivation, immobilized cells produced an appreciable level of xylanase ac...

journal_title：Applied biochemistry and biotechnology

authors： Mamo G,Gessesse A

更新日期：2000-05-01 00:00:00

abstract：:Ethanol production was studied in simultaneous saccharification and fermentation (SSF) of steam-pretreated spruce at 42 degrees C, using a thermotolerant yeast. Three yeast strains of Kluyveromyces marxianus were compared in test fermentations. SSF experiments were performed with the best of these on 5% (w/w) of subst...

journal_title：Applied biochemistry and biotechnology

authors： Bollók M,Réczey K,Zacchi G

更新日期：2000-04-01 00:00:00

abstract：:Possible methods to minimize the toxic effects of SOx and NOx on the growth of a highly CO2 tolerant and fast-growing microalga, Chlorella sp. KR-1, were investigated. Maintaining the pH of the culturing media at an adequate value was quite important to enhancing the tolerances of the microalgae to SOx and NOx. Contro...

journal_title：Applied biochemistry and biotechnology

authors： Lee JN,Lee JS,Shin CS,Park SC,Kim SW

更新日期：2000-04-01 00:00:00

abstract：:We report the identification of five proteins expressed specifically on the bacterial magnetic particle (BMP) membrane of Magnetospirillum sp. AMB-1. These proteins are major components of the BMP membrane. The molecular weights were determined to be 12.0, 16.0, 24.8, 35.6, and 66.2 kDa by sodium dodecyl sulfate polya...

journal_title：Applied biochemistry and biotechnology

authors： Okamura Y,Takeyama H,Matsunaga T

更新日期：2000-04-01 00:00:00

abstract：:In this study, we investigated the dynamics of a computer simulation of a continuous alcoholic fermentation process combined with a flash column under vacuum. The alcohol was partially extracted in order to maintain its concentration at about 40 kg/m3 in the fermentor. The mathematical model of the fermentation was de...

journal_title：Applied biochemistry and biotechnology

authors： Costa AC,Dechechi EC,Silva FL,Maugeri F,Maciel R

更新日期：2000-04-01 00:00:00

abstract：:The microheterogeneous native amylolytic complex secreted by the isolate A6 of Lactobacillus plantarum revealed a selective enzyme specificity loss when submitted to a limited proteolysis under a suboptimum pH condition. A clear electrophoretic profile change toward just one shorter, more acidic, and equally active po...

journal_title：Applied biochemistry and biotechnology

authors： Florêncio JA,Eiras-Stofella DR,Soccol CR,Raimbault M,Guyot JP,Fontana JD

更新日期：2000-04-01 00:00:00

abstract：:The production of cellulase-free endoxylanase by the thermophilic fungus Thermomyces lanuginosus was investigated in semisolid fermentation and liquid fermentation. Different process variables were investigated in semisolid fermentation, employing corncob as the carbon source. The best results were with the following ...

journal_title：Applied biochemistry and biotechnology

authors： Damaso MC,Andrade CM,Pereira Júnior N

更新日期：2000-04-01 00:00:00

abstract：:A strain of Clostridium thermoaceticum (ATCC 49707) was evaluated for its homoacetate potential. This thermophilic anaerobe best produces acetate from glucose at pH 6.0 and 59 degrees C with a yield of 83% of theoretical. Enzyme hydrolysis of two substrates, a-cellulose and a pulp mill sludge, yielded 68% and 70% dige...

journal_title：Applied biochemistry and biotechnology

authors： Borden JR,Lee YY,Yoon HH

更新日期：2000-04-01 00:00:00

abstract：:A simple staged model for the protein foam fractionation process is proposed in this article. This simplified model does not detail the complex foam structure and gas-liquid hydrodynamics in the foam phase but, rather, is built on the conventional theoretical stage concept considering upward bubbles with entrained liq...

journal_title：Applied biochemistry and biotechnology

authors： Du L,Loha V,Tanner RD

更新日期：2000-04-01 00:00:00

abstract：:Feather waste, generated in large quantities as a byproduct of commercial poultry processing, is almost pure keratin, which is not easily degradable by common proteolytic enzymes. Feather-degrading bacteria were isolated from a Brazilian poultry industrial waste. Among these isolates, a strain identified as kr2 was th...

journal_title：Applied biochemistry and biotechnology

authors： Sangali S,Brandelli A

更新日期：2000-04-01 00:00:00